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- Q & A: 204 Questions and Answers
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American Society of Microbiology ABMM Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Directing Laboratory Administrative Functions | 19% | - Personnel management, competency assessment and training - Process improvement and risk management - Laboratory operations, budgeting and compliance - Accreditation, licensure and regulatory requirements - Information management and reporting |
| Topic 2: Ensuring Safety and Security in the Laboratory | 11.5% | - Biological, chemical and physical safety - Security, waste management and emergency response |
| Topic 3: Directing Laboratory Testing Functions | 44.5% | - Test selection, validation, quality control and interpretation - Up-to-date practices, standards, technologies and emerging diseases |
| Topic 4: Consulting with Other Medical Professionals | 25% | - Clinical consultation, result interpretation and guidance - Education, research and professional development - Public health, epidemiology and outbreak investigation |
American Society of Microbiology ABMM Sample Questions:
Question #1
A 55-year-old male with a history of poorly controlled diabetes mellitus develops a necrotizing fasciitis of his left lower extremity following a minor abrasion sustained while gardening. Initialcultures of the wound reveal a polymicrobial flora, including Gram-positive cocci in chains and Gram-negative bacilli. Over the next 48 hours, despite broad-spectrum antibiotic therapy, the patient's condition deteriorates, and new bullae with hemorrhagic fluid appear. Gram stain of this fluid now predominantly shows large, Gram-positive bacilli with subterminal spores. Which of the following virulence factors is MOST likely contributing to the rapid tissue destruction observed in this patient?
- A. Lecithinase (alpha-toxin)
- B. Lipopolysaccharide (LPS)
- C. Exotoxin B (SpeB)
- D. Protein A
Answer: A
Question #2
A patient with severe combined immunodeficiency (SCID) develops a progressive respiratory illness.
Bronchoalveolar lavage fluid is examined using direct fluorescent antibody (DFA) staining, which reveals numerous cysts of Pneumocystis jirovecii. The cysts appear:
- A. Large and multinucleated with prominent endospores.
- B. Oval-shaped with a thin wall and internal nuclei.
- C. Cup-shaped or helmet-shaped with internal structures.
- D. Small and round with a thick wall, containing sporozoites.
Answer: C
Question #3
A traveler returning from sub-Saharan Africa presents with cyclical fevers, chills, and sweats. A Giemsa- stained peripheral blood smear reveals multiple ring forms within red blood cells, some applique forms, and occasional banana-shaped gametocytes. No Schüffner's dots or red blood cell enlargement is observed. Which Plasmodium species is the most likely cause?
- A. Plasmodium ovale
- B. Plasmodium vivax
- C. Plasmodium malariae
- D. Plasmodium falciparum
Answer: D
Question #4
The direct fluorescent antibody (DFA) test is commonly used for the rapid detection of which respiratory pathogen directly from nasopharyngeal specimens?
- A. Bordetella pertussis
- B. Mycoplasma pneumoniae
- C. Legionella pneumophila (serogroup 1 in sputum)
- D. Influenza virus
Answer: A
Question #5
A patient with a history of recurrent urinary tract infections caused by Escherichia coli develops a new infection. The urine isolate is resistant to trimethoprim-sulfamethoxazole. The MOST common mechanism of resistance to this antibiotic combination in E. coli involves:
- A. Increased production of para-aminobenzoic acid (PABA).
- B. Expression of efflux pumps that specifically target trimethoprim and sulfamethoxazole.
- C. Enzymatic inactivation of trimethoprim and sulfamethoxazole.
- D. Mutations in the genes encoding dihydrofolate reductase and dihydropteroate synthase.
Answer: D
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